VAV3 Antibody (9A10D8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16615

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16615-100ul
NBP3-16615-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9A10D8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all VAV3 Antibodies »

Product Summary for VAV3 Antibody (9A10D8)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 700-800 of human VAV3 (Q9UKW4). TKESGEYAISIKYNNEAKHIKILTRDGFFHIAENRKFKSLMELVEYYKHHSLKEGFRTLDTTLQFPYKEPEHSAGQRGNRAGNSLLSPKVLGIAIARYDFC

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VAV3 Antibody (9A10D8)

Western Blot: VAV3 Antibody (9A10D8) [NBP3-16615]

Western Blot: VAV3 Antibody (9A10D8) [NBP3-16615]

Western Blot: VAV3 Antibody (9A10D8) [NBP3-16615] - Analysis of extracts of various cell lines, using VAV3 antibody (NBP3-16615) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: VAV3 Antibody (9A10D8) [NBP3-16615]

Immunohistochemistry-Paraffin: VAV3 Antibody (9A10D8) [NBP3-16615]

Immunohistochemistry-Paraffin: VAV3 Antibody (9A10D8) [NBP3-16615] - Human esophageal cancer using VAV3 Rabbit mAb (NBP3-16615) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for VAV3 Antibody (9A10D8)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:100 - 1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VAV3

The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav) (1). Vav3 is expressed in most hematopoietic cells and non-hematopoietic tissues except ovary and skeletal muscles. T-cell stimulated and tyrosine phosphorylated Vav3 acts as an exchange factor for GTP-binding proteins RhoA, RhoG, and Rac1 (2). Also, tyrosine phosphorylated Vav3 mediates RTK signalizing, specifically IR, IGFR, and EGFR-mediated signaling pathways. Vav3 has been linked to as a factor for cell morphology modulation and an inducer for cell transformation (3).

Alternate Names

FLJ40431, guanine nucleotide exchange factor VAV3, vav 3 guanine nucleotide exchange factor, vav 3 oncogene, VAV-3

Gene Symbol

VAV3

Additional VAV3 Products

Product Documents for VAV3 Antibody (9A10D8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VAV3 Antibody (9A10D8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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