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VAV3 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93949

Novus Biologicals, part of Bio-Techne
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NBP2-93949-0.02ml
NBP2-93949-0.1ml

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for VAV3 Antibody - Azide and BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 560-680 of human VAV3 (NP_006104.4). DNCGRVNSGEQGTLKLPEKRTNGLRRTPKQVDPGLPKMQVIRNYSGTPPPALHEGPPLQLQAGDTVELLKGDAHSLFWQGRNLASGEVGFFPSDAVKPCPCVPKPVDYSCQPWYAGAMERL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VAV3 Antibody - Azide and BSA Free

Western Blot: VAV3 AntibodyAzide and BSA Free [NBP2-93949]

Western Blot: VAV3 AntibodyAzide and BSA Free [NBP2-93949]

Western Blot: VAV3 Antibody [NBP2-93949] - Analysis of extracts of various cell lines, using VAV3 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 2s.
Immunocytochemistry/ Immunofluorescence: VAV3 Antibody - Azide and BSA Free [NBP2-93949]

Immunocytochemistry/ Immunofluorescence: VAV3 Antibody - Azide and BSA Free [NBP2-93949]

Immunocytochemistry/Immunofluorescence: VAV3 Antibody [NBP2-93949] - Analysis of L929 cells using VAV3 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: VAV3 Antibody - Azide and BSA Free [NBP2-93949]

Immunocytochemistry/ Immunofluorescence: VAV3 Antibody - Azide and BSA Free [NBP2-93949]

Immunocytochemistry/Immunofluorescence: VAV3 Antibody [NBP2-93949] - Analysis of HeLa cells using VAV3 at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for VAV3 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:200-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VAV3

The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav) (1). Vav3 is expressed in most hematopoietic cells and non-hematopoietic tissues except ovary and skeletal muscles. T-cell stimulated and tyrosine phosphorylated Vav3 acts as an exchange factor for GTP-binding proteins RhoA, RhoG, and Rac1 (2). Also, tyrosine phosphorylated Vav3 mediates RTK signalizing, specifically IR, IGFR, and EGFR-mediated signaling pathways. Vav3 has been linked to as a factor for cell morphology modulation and an inducer for cell transformation (3).

Alternate Names

FLJ40431, guanine nucleotide exchange factor VAV3, vav 3 guanine nucleotide exchange factor, vav 3 oncogene, VAV-3

Gene Symbol

VAV3

Additional VAV3 Products

Product Documents for VAV3 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VAV3 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov