VE-Cadherin Antibody (BV9)
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1409
Clone BV9 was used by HLDA to establish CD designation.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Functional, Immunoassay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # BV9
Concentration
0.1 mg/ml
Product Specifications
Immunogen
The antibody recognizes the extracellular domain of the protein.
Localization
Intercellular junctions
Specificity
This monoclonal antibody (subclass IgG2a) binds to human VE-cadherin. Human vascular endothelium cadherin is an endothelium-specific cadherin localized at the intercellular junctions.
Marker
Endothelial Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for VE-Cadherin Antibody (BV9)
Immunocytochemistry/ Immunofluorescence: VE-Cadherin Antibody (BV9) [NB600-1409]
Immunocytochemistry/Immunofluorescence: VE Cadherin Antibody (BV9) [NB600-1409] - Endothelial cells were fixed with 4% PAF (15 min, room temp), and then permeabilized with 0.5% TritionX-100 (3 min, room temperature). Cells were incubated with a final concentration of 10mg/ml BV9. Secondary detection was performed with anti-mouse Alexa-Fluor 647 and counterstained using DAPI.Flow Cytometry: VE-Cadherin Antibody (BV9) [NB600-1409]
Flow Cytometry: VE Cadherin Antibody (BV9) [NB600-1409] - HUVEC cells were stained with antibody BV9 in PBS/0.1% saponin for 1h at 4 degrees C.(Black- no stain, Red- isotype control, Blue-HM2032-BV9)Applications for VE-Cadherin Antibody (BV9)
Application
Recommended Usage
Flow Cytometry
1:10-1:100
Functional
10-50 ug/ml
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:10-1:100
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS with 0.1% BSA
Preservative
No Preservative
Concentration
0.1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: VE-Cadherin
Long Name
Vascular Endothelium Cadherin
Alternate Names
Cadherin-5, CD144, CDH5, VECadherin
Entrez Gene IDs
1003 (Human)
Gene Symbol
CDH5
UniProt
Additional VE-Cadherin Products
Product Documents for VE-Cadherin Antibody (BV9)
Product Specific Notices for VE-Cadherin Antibody (BV9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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