VEGFR1/Flt-1 Antibody (EWC) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1004

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, In vivo assay, Western Blot

Cited:

In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # EWC

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all VEGFR1/Flt-1 Antibodies »

Product Specifications

Immunogen

Recombinant human soluble extracellular Flt-1 (D5)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Type I membrane protein (Flt1) and soluble (SFlt1).

Specificity

The monoclonal will detect native and denaturated human VEGFR-1/ Flt-1 in ELISA experiments and on the surface of different human cell types. The will also detect mouse Flt-1.

Marker

Endothelial Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for VEGFR1/Flt-1 Antibody (EWC) - Azide and BSA Free

Western Blot: VEGFR1/Flt-1 Antibody (EWC)Azide and BSA Free [NB600-1004]

Western Blot: VEGFR1/Flt-1 Antibody (EWC)Azide and BSA Free [NB600-1004]

Western Blot: VEGFR1/Flt-1 Antibody (Flt-1/EWC) [NB600-1004] - Analysis of recombinant human and mouse soluble VEGF receptors using a monoclonal antibody directed against human recombinant sFlt-1(D5). There is a cross reactivity with mouse sFlt-1(D7)-Fc but no with human and mouse sKDR and human sFLT-4 visible.
Immunocytochemistry/ Immunofluorescence: VEGFR1/Flt-1 Antibody (EWC) - Azide and BSA Free [NB600-1004]

Immunocytochemistry/ Immunofluorescence: VEGFR1/Flt-1 Antibody (EWC) - Azide and BSA Free [NB600-1004]

Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody (EWC) - Azide and BSA Free [NB600-1004] - VEGFR1/Flt-1 Antibody (Flt-1/EWC) [NB600-1004] - Staining of human Flt-1 in a co-culture of PAE-Flt-1, PAE-KDR and PAE-FLT-4 with a mouse anti-human Flt-1 antibody. Conjugated secondary antibody: goat anti-mouse ALEXA Flour (1:600).
Western Blot: VEGFR1/Flt-1 Antibody (EWC)Azide and BSA Free [NB600-1004]

Western Blot: VEGFR1/Flt-1 Antibody (EWC)Azide and BSA Free [NB600-1004]

Western Blot: VEGFR1/Flt-1 Antibody (Flt-1/EWC) [NB600-1004] - Analysis of recombinant mouse soluble VEGFR-1 conditioned supernatant using a monoclonal antibody directed against human recombinant sFlt-1(D5). There is a strong cross reactivity with the mouse sFlt-1 visible.

Applications for VEGFR1/Flt-1 Antibody (EWC) - Azide and BSA Free

Application
Recommended Usage

ELISA

1-10 ug/ml

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Western Blot

2-5 ug/ml
Application Notes
Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Use In vivo reported in scientific literature (PMID:31877123)

Reviewed Applications

Read 3 reviews rated 4.3 using NB600-1004 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VEGFR1/Flt-1

VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity. Because sFLT1 has a higher affinity for VEGF than does FLK1, it may function as an inhibitor of VEGF response.

Long Name

Vascular Endothelial Growth Factor Receptor 1

Alternate Names

Flt-1, FLT1, FRT, VEGF R1, VEGFR-1

Gene Symbol

FLT1

Additional VEGFR1/Flt-1 Products

Product Documents for VEGFR1/Flt-1 Antibody (EWC) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VEGFR1/Flt-1 Antibody (EWC) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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