VEGFR1/Flt-1 Antibody (EWC) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-18636

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # EWC

Concentration

LYOPH mg/ml

View all VEGFR1/Flt-1 Antibodies »

Product Specifications

Immunogen

Recombinant human soluble extracellular Flt-1 (D5)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Type I membrane protein (Flt1) and soluble (SFlt1).

Specificity

The monoclonal will detect native and denaturated human VEGFR-1/ Flt-1 in ELISA experiments and on the surface of different human cell types. The will also detect mouse Flt-1.

Marker

Endothelial Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for VEGFR1/Flt-1 Antibody (EWC) [Biotin]

Application
Recommended Usage

ELISA

2.5 ug/ml

Immunoprecipitation

1-5 ug/ml

Western Blot

2-5 ug/ml
Application Notes
This is useful for the following applications: ELISA, Western blotting, immunofluorescence, and immunoprecipitation.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Formulation

PBS; 50X BSA

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VEGFR1/Flt-1

VEGF Receptor 1 (also known as FLT) belongs to the src gene family and shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The protein acts as a receptor for VEGF, VEGFB and PGF. An alternatively spliced form of the gene produces a soluble protein (sFlt1) which binds vascular endothelial growth factor (VEGF) with high affinity. sFlt1 has a higher affinity for VEGF indicating that it may function as an inhibitor in the VEGF response. VEGF Receptor 1 is specifically expressed in most vascular endothelial cells and peripheral blood monocytes.

Long Name

Vascular Endothelial Growth Factor Receptor 1

Alternate Names

Flt-1, FLT1, FRT, VEGF R1, VEGFR-1

Gene Symbol

FLT1

Additional VEGFR1/Flt-1 Products

Product Documents for VEGFR1/Flt-1 Antibody (EWC) [Biotin]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VEGFR1/Flt-1 Antibody (EWC) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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