Viral EBV IL-10 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB915
Key Product Details
Species Reactivity
Validated:
Viral
Cited:
Virus
Applications
Validated:
Neutralization, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 134216
Product Specifications
Immunogen
E. coli-derived recombinant viral EBV IL-10
Gln26-Arg170
Accession # P03180
Gln26-Arg170
Accession # P03180
Specificity
Detects viral EBV IL‑10 in direct ELISAs and Western blots. In Western blots, less than 10% cross-reactivity with recombinant canine IL-10, recombinant feline IL-10, recombinant mouse IL-10, recombinant porcine IL-10, and recombinant rat IL-10 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Viral EBV IL-10 Antibody
Cell Proliferation Induced by IL‑10 and Neutralization by Viral IL‑10 Antibody.
Recombinant Viral EBV IL-10 (Catalog # 915-VL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Viral EBV IL-10 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Viral EBV IL-10 Monoclonal Antibody (Catalog # MAB915). The ND50 is typically 0.1-0.4 µg/mL.Applications for Viral EBV IL-10 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Viral EBV IL-10 (Catalog # 915-VL)
Sample: Recombinant Viral EBV IL-10 (Catalog # 915-VL)
Neutralization
Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. Thompson‑Snipes, L. et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND50) is typically 0.1‑0.4 µg/mL in the presence of 100 ng/mL Recombinant Viral EBV IL‑10.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-10
Interleukin 10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.
Long Name
Interleukin 10
Alternate Names
CSIF, GVHDS, IL10, IL10A, TGIF
Entrez Gene IDs
Gene Symbol
IL10
UniProt
Additional IL-10 Products
Product Documents for Viral EBV IL-10 Antibody
Product Specific Notices for Viral EBV IL-10 Antibody
For research use only
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