Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1E1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

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Product Summary for Von Hippel Lindau Antibody (OTI1E1) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human VHL(NP_000542) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Von Hippel Lindau Antibody (OTI1E1) - Azide and BSA Free

Western Blot: Von Hippel Lindau Antibody (OTI1E1)Azide and BSA Free [NBP2-74851]

Western Blot: Von Hippel Lindau Antibody (OTI1E1)Azide and BSA Free [NBP2-74851]

Western Blot: Von Hippel Lindau Antibody (OTI1E1) - Azide and BSA Free [NBP2-74851] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Von Hippel Lindau.
Immunohistochemistry: Von Hippel Lindau Antibody (OTI1E1) - Azide and BSA Free [NBP2-74851]

Immunohistochemistry: Von Hippel Lindau Antibody (OTI1E1) - Azide and BSA Free [NBP2-74851]

Immunohistochemistry: Von Hippel Lindau Antibody (OTI1E1) - Azide and BSA Free [NBP2-74851] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Von Hippel Lindau Antibody (OTI1E1) - Azide and BSA Free [NBP2-74851]

Immunohistochemistry: Von Hippel Lindau Antibody (OTI1E1) - Azide and BSA Free [NBP2-74851]

Immunohistochemistry: Von Hippel Lindau Antibody (OTI1E1) - Azide and BSA Free [NBP2-74851] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for Von Hippel Lindau Antibody (OTI1E1) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:4000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VHL

Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Long Name

Von Hippel-Lindau Tumor Suppressor

Alternate Names

Protein G7, pVHL

Gene Symbol

VHL

Additional VHL Products

Product Documents for Von Hippel Lindau Antibody (OTI1E1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Von Hippel Lindau Antibody (OTI1E1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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