Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all WASP Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, is phosphorylated Serine 483, phosphorylated Serine 484 and surrounding residues of human Wiskott-Aldrich syndrome using the numbering given in entry NP_000368.1 (GeneID 7454).

Modification

p Ser483, p Ser484

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WASP [p Ser483, p Ser484] Antibody

Western Blot: WASP [p Ser483, p Ser484] Antibody [NB100-2307]

Western Blot: WASP [p Ser483, p Ser484] Antibody [NB100-2307]

Western Blot: WASP [p Ser483, p Ser484] Antibody [NB100-2307] - Detection of Human Phospho WASP (S483/S484) by Western Blot. Samples: Whole cell lysate (50 ug) from Jurkat and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho WASP (S483/S484) antibody NB100-2307 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for WASP [p Ser483, p Ser484] Antibody

Application
Recommended Usage

Western Blot

1:1000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: WASP

The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known.

Long Name

Wiskott-Aldrich Syndrome Protein

Alternate Names

Thrombocytopenia 1, WAS

Entrez Gene IDs

7454 (Human)

Gene Symbol

WAS

UniProt

P42768

Additional WASP Products

Product Documents for WASP [p Ser483, p Ser484] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WASP [p Ser483, p Ser484] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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