Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Wnt-10b Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid synthetic peptide from near the center of human Wnt10b. The immunogen is located within amino acids 130 - 180 of Wnt10b. Amino Acid Squence: CGWKGSGEQDRLRAK

Specificity

Two isoforms of Wnt10b are known to exist; this antibody will only recognize the longer isoform. This Wnt10b antibody will not cross-react with Wnt10a.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Wnt-10b Antibody - BSA Free

Western Blot: Wnt-10b AntibodyBSA Free [NBP1-77325]

Western Blot: Wnt-10b AntibodyBSA Free [NBP1-77325]

Western Blot: Wnt-10b Antibody [NBP1-77325] - human skeletal muscle tissue lysate with Wnt10b antibody at (A) 2 and (B) 4 ug/ml.
Immunohistochemistry-Paraffin: Wnt-10b Antibody - BSA Free [NBP1-77325]

Immunohistochemistry-Paraffin: Wnt-10b Antibody - BSA Free [NBP1-77325]

Immunohistochemistry-Paraffin: Wnt-10b Antibody [NBP1-77325] - Human skeletal muscle with Wnt10b antibody at 2.5 ug/mL.

Applications for Wnt-10b Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

2-4 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Wnt-10b

Wnt10b is a member of the Wnt family, a gene family that encodes secreted signaling proteins that play crucial roles in normal development such as regulation of cell fate and patterning during embryogenesis as well as neoplastic transformation. Wnt10b is found in the mouse mammary tumor virus insertion site where it is activated and causes mammary tumors. Elevated levels of Wnt10b have also been detected in human breast carcinomas. Wnt10b is known to be involved in adipogenesis, maintaining the preadipocyte in an undifferentiated state. More recently, Wnt10b has been shown to promote epithelial cell differentiation and hair shaft growth, demonstrating that Wnt10b has multiple roles in cell growth and differentiation. Two isoforms of Wnt10b are known to exist; this antibody will only recognize the longer isoform. This Wnt10b antibody will not cross-react with Wnt10a.

Long Name

Wingless-type MMTV Integration Site Family, Member 10B

Alternate Names

Wnt-12, Wnt10b

Gene Symbol

WNT10B

UniProt

NP_003385

Additional Wnt-10b Products

Product Documents for Wnt-10b Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Wnt-10b Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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