YB1 Antibody (rYBX1/2430)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07461
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: YB1 Antibody (rYBX1/2430) [NBP3-07461]](https://resources.bio-techne.com/images/products/YB1-Antibody-rYBX1-2430-Immunohistochemistry-Paraffin-NBP3-07461-img0001.jpg "Immunohistochemistry-Paraffin: YB1 Antibody (rYBX1/2430) [NBP3-07461]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # rYBX1/2430
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human full-length YB1 protein (Uniprot: P67809)
Localization
Cytoplasmic and Nuclear
Specificity
The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Y-box binding protein-1 (YBX1) is the prototypic member of the cold shock protein family that fulfills numerous cellular functions. In the nucleus, YBX1 protein orchestrates transcription of proliferation-related genes, whereas in the cytoplasm it associates with mRNA and directs translation. In human tumor entities, such as breast, lung and prostate cancer, cellular YBX1 expression indicates poor clinical outcome, suggesting that YBX1 is an attractive marker to predict patients prognosis.
Marker
Tumor Biomarker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08783)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for YB1 Antibody (rYBX1/2430)
Immunohistochemistry-Paraffin: YB1 Antibody (rYBX1/2430) [NBP3-07461]
Immunohistochemistry-Paraffin: YB1 Antibody (rYBX1/2430) [NBP3-07461] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with YB1 Mouse Recombinant Monoclonal Antibody (rYB1/2430).Applications for YB1 Antibody (rYBX1/2430)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: YB1
Alternate Names
BP-8, CBF-A, class II, Y box-binding protein I, CSDA2, CSDB, DBPB CCAAT-binding transcription factor I subunit A, EFI-A, Enhancer factor I subunit A, MDR-NF1, MGC104858, MGC110976, MGC117250, NSEP1 Y-box-binding protein 1, nuclease-sensitive element-binding protein 1, Y box binding protein 1, YB1 DNA-binding protein B, YB-1 Y-box transcription factor, YBX1
Gene Symbol
YBX1
UniProt
Additional YB1 Products
Product Documents for YB1 Antibody (rYBX1/2430)
Product Specific Notices for YB1 Antibody (rYBX1/2430)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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