ZAP70 Antibody (RM408)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89104

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-89104
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM408

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ZAP70 Antibodies »

Product Specifications

Immunogen

A peptide corresponding to residues near the N-terminus of human ZAP70

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZAP70 Antibody (RM408)

Western Blot: ZAP70 Antibody (RM408) [NBP2-89104]

Western Blot: ZAP70 Antibody (RM408) [NBP2-89104]

Western Blot: ZAP70 Antibody (RM408) [NBP2-89104] - Western Blot of Jurkat cell lysate using Anti-ZAP70 rabbit monoclonal antibody (Clone RM408) at a 1:500 dilution.
Immunohistochemistry-Paraffin: ZAP70 Antibody (RM408) [NBP2-89104]

Immunohistochemistry-Paraffin: ZAP70 Antibody (RM408) [NBP2-89104]

Immunohistochemistry-Paraffin: ZAP70 Antibody (RM408) [NBP2-89104] - Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using Anti-ZAP70 Rabbit Monoclonal Antibody (Clone RM408) at a 1:200 dilution.

Applications for ZAP70 Antibody (RM408)

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ZAP70

Zap-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation (1). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with activation of ZAP-70, a critical event in T cell activation (2). Following TCR engagement, ZAP-70 is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family tyrosine kinase, Lck. Phosphorylation of Tyr319 is required for full activation and increased positive downstream regulation by ZAP-70 (3).

Long Name

Zeta-chain [TCR] Associated Protein Kinase 70 kDa

Alternate Names

mrtle, mur, SRK, STD, TZK

Gene Symbol

ZAP70

Additional ZAP70 Products

Product Documents for ZAP70 Antibody (RM408)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ZAP70 Antibody (RM408)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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