ZAP70 Antibody (ZAP70/4040R) [Janelia Fluor® 646]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08968JF646

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry-Paraffin

Label

Janelia Fluor 646

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # ZAP70/4040R

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ZAP70 Antibodies »

Product Summary for ZAP70 Antibody (ZAP70/4040R) [Janelia Fluor® 646]

Immunogen

Synthetic peptide corresponding to residues withinaa200-350 of ZAP70 was used as an immunogen. (Uniprot: P43403 )

Localization

Cell surface and cytoplasm

Marker

Chronic Lymphocytic Leukemia Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for ZAP70 Antibody (ZAP70/4040R) [Janelia Fluor® 646]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: ZAP70

Zap-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation (1). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with activation of ZAP-70, a critical event in T cell activation (2). Following TCR engagement, ZAP-70 is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family tyrosine kinase, Lck. Phosphorylation of Tyr319 is required for full activation and increased positive downstream regulation by ZAP-70 (3).

Long Name

Zeta-chain [TCR] Associated Protein Kinase 70 kDa

Alternate Names

mrtle, mur, SRK, STD, TZK

Gene Symbol

ZAP70

Additional ZAP70 Products

Product Documents for ZAP70 Antibody (ZAP70/4040R) [Janelia Fluor® 646]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ZAP70 Antibody (ZAP70/4040R) [Janelia Fluor® 646]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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