ZAP70 [p Tyr319] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-18896

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ZAP70 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region including tyrosine 319 (when it is phosphorylated) of human zeta-chain (TCR) associated protein kinase 70kDa using the numbering given in entry NP_001070.2 (GeneID 7535).

Modification

p Tyr319

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZAP70 [p Tyr319] Antibody

Flow Cytometry: ZAP70 [p Tyr319] Antibody [NBP1-18896]

Flow Cytometry: ZAP70 [p Tyr319] Antibody [NBP1-18896]

Flow Cytometry: ZAP70 [p Tyr319] Antibody [NBP1-18896] - Flow cytometric analysis of phospho-Zap70 (Y319). Jurkat cells were treated with H2O2 for 3 minutes. Cells were fixed in 1.5% PFA, permeabilized in 90%, and 1 X 10e6 cells stained with 1.0 mcg/ml Phospho Zap70 (Y319) antibody in a 150 mcl volume. Isotype anti-KLH control (black) in H2O2 treated cells, anti-phospho Zap70 (Y319) in untreated cells (red), antiphospho Zap70 (Y319) in H2O2 treated (blue).
Immunoprecipitation: ZAP70 [p Tyr319] Antibody [NBP1-18896]

Immunoprecipitation: ZAP70 [p Tyr319] Antibody [NBP1-18896]

Immunoprecipitation: ZAP70 [p Tyr319] Antibody [NBP1-18896] - Whole cell lysate from Jurkat cells that had been treated with hydrogen peroxide. ZAP70 antibodies NBP1-18896, NBP1-1889, and NBP1-18898 used for IP at 3 mcg/mg lysate.
Immunoprecipitation: ZAP70 [p Tyr319] Antibody [NBP1-18896]

Immunoprecipitation: ZAP70 [p Tyr319] Antibody [NBP1-18896]

Immunoprecipitation: ZAP70 [p Tyr319] Antibody [NBP1-18896] - Jurkat WC Lysate treated with hydrogen peroxide (+) or mock treated (-). NB100-97826 was used to IP ZAP70. The IPs were either treated with phosphatase (+) or mock (-) treated.

Applications for ZAP70 [p Tyr319] Antibody

Application
Recommended Usage

Flow Cytometry

10 ug per 1 X 10e6 cells in a 150mcl volume

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Western Blot Not recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ZAP70

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Zeta-chain [TCR] Associated Protein Kinase 70 kDa

Alternate Names

mrtle, mur, SRK, STD, TZK

Entrez Gene IDs

7535 (Human)

Gene Symbol

ZAP70

UniProt

NP_001070.2 (Human)

Additional ZAP70 Products

Product Documents for ZAP70 [p Tyr319] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZAP70 [p Tyr319] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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