Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1F3

Concentration

0.5 mg/ml

View all ZNF397 Antibodies »

Product Summary for ZNF397 Antibody (OTI1F3)

Immunogen

Full length human recombinant protein of human ZNF397(NP_115723) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

30.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ZNF397 Antibody (OTI1F3)

Western Blot: ZNF397 Antibody (OTI1F3) [NBP2-01324]

Western Blot: ZNF397 Antibody (OTI1F3) [NBP2-01324]

Western Blot: ZNF397 Antibody (1F3) [NBP2-01324] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF397 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF397.
Immunocytochemistry/Immunofluorescence: ZNF397 Antibody (1F3) [NBP2-01324] Staining of COS7 cells transiently transfected by pCMV6-ENTRY ZNF397.

Applications for ZNF397 Antibody (OTI1F3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ZNF397

This gene encodes a protein with a N-terminal SCAN domain, and the longer isoform contains nine C2H2-type zinc finger repeats in the C-terminal domain. The protein localizes to centromeres during interphase and early prophase, and different isoforms can repress or activate transcription in transfection studies. Multiple transcript variants encoding different isoforms have been found for this gene. Additional variants have been described, but their biological validity has not been determined. [provided by RefSeq].

Alternate Names

KIAA1588, KRAB-containing zinc finger protein 317, zinc finger protein 317

Entrez Gene IDs

84307 (Human)

Gene Symbol

ZNF397

Additional ZNF397 Products

Product Documents for ZNF397 Antibody (OTI1F3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ZNF397 Antibody (OTI1F3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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