Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1F3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ZNF397 Antibodies »

Product Summary for ZNF397 Antibody (OTI1F3) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human ZNF397(NP_115723) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

30.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ZNF397 Antibody (OTI1F3) - Azide and BSA Free

Western Blot: ZNF397 Antibody (OTI1F3)Azide and BSA Free [NBP2-74950]

Western Blot: ZNF397 Antibody (OTI1F3)Azide and BSA Free [NBP2-74950]

Western Blot: ZNF397 Antibody (OTI1F3) - Azide and BSA Free [NBP2-74950] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF397 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF397.
Immunocytochemistry/ Immunofluorescence: ZNF397 Antibody (OTI1F3) - Azide and BSA Free [NBP2-74950]

Immunocytochemistry/ Immunofluorescence: ZNF397 Antibody (OTI1F3) - Azide and BSA Free [NBP2-74950]

Immunocytochemistry/Immunofluorescence: ZNF397 Antibody (OTI1F3) - Azide and BSA Free [NBP2-74950] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ZNF397.

Applications for ZNF397 Antibody (OTI1F3) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ZNF397

The ZNF397 gene encodes a protein with a N-terminal SCAN domain, and the longer isoform contains nine C2H2-type zinc fingerrepeats in the C-terminal domain. The protein localizes to centromeres during interphase and early prophase, anddifferent isoforms can rep

Alternate Names

KIAA1588, KRAB-containing zinc finger protein 317, zinc finger protein 317

Gene Symbol

ZNF397

Additional ZNF397 Products

Product Documents for ZNF397 Antibody (OTI1F3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZNF397 Antibody (OTI1F3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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