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Mouse Caspase-9 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75042

Novus Biologicals, part of Bio-Techne
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NBP2-75042

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse CASP9 in samples. No significant cross-reactivity or interference between Mouse CASP9 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.39%

Inter-Assay Precision (Precision between assays) CV% < 5.31%

Recovery for Mouse Caspase-9 ELISA Kit (Colorimetric)

Recovery

87-106%

Linearity

Mouse Caspase-9 ELISA Kit (Colorimetric)

Scientific Data Images for Mouse Caspase-9 ELISA Kit (Colorimetric)

Mouse Caspase-9 ELISA Kit (Colorimetric)

ELISA: Mouse Caspase-9 ELISA Kit (Colorimetric) [NBP2-75042] -

ELISA: Mouse Caspase-9 ELISA Kit (Colorimetric) [NBP2-75042] - Standard Curve Reference

Kit Contents for Mouse Caspase-9 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Caspase-9

Caspase 9 (CASP9) is a member of the cysteine-aspartic acid protease (caspase) family that is involved in the execution-phase of cell apoptosis. Caspase 9 is expressed as an inactive proenzyme which then undergos proteolytic processing by upstream caspases, to form an active (cleaved) caspase 9 form.

There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Caspase 3 is considered an initiator, as initiated caspase-9 goes on to cleave the effector procaspase-3 and procaspase-7.

Caspase 9 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.

Alternate Names

CASP9, Caspase9

Gene Symbol

CASP9

Additional Caspase-9 Products

Product Documents for Mouse Caspase-9 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse Caspase-9 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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