Rat E-Selectin/CD62E ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80383

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum (100 ul)

Sensitivity

3.78 pg/ml

Assay Range

23.44-1500 pg/ml (example only; lot dependent)

View all E-Selectin/CD62E ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Conjugate

HRP

Reactivity

Rat

Specificity

Recognizes both recombinant and natural Rat E-Selectin/CD62E.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 3.1

Inter-Assay Precision (Precision between assays) CV% < 5.6 %

Recovery for Rat E-Selectin/CD62E ELISA Kit (Colorimetric)

Recovery

80-88%

Scientific Data Images for Rat E-Selectin/CD62E ELISA Kit (Colorimetric)

Sandwich ELISA: Rat E-Selectin/CD62E ELISA Kit (Colorimetric) [NBP2-80383] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Kit Contents for Rat E-Selectin/CD62E ELISA Kit (Colorimetric)

  • 1. 96 well microplate coated with Capture Antibody
  • 2. Detection Antibody conjugated to HRP
  • 3. Standards
  • 4. Wash Buffer Concentrate
  • 5. Dilution Buffer Concentrate
  • 6. Color Reagent A
  • 7. Color Reagent B
  • 8. Stop Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage is content dependent.

Background: E-Selectin/CD62E

CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I membrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells (IL-2, TNF- alpha, other cytokines can increase expression) and can also be expressed on endothelial cells in the skin, bone marrow and placenta. CD62E is involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Siayl Lewis X (CD15s) and PSGL-1 (CD162). The HCD62E antibody has been shown to recognize human CD62E and to be useful for flow cytometry.

Alternate Names

CD62E, ELAM1, LECAM2, SELE

Gene Symbol

SELE

Additional E-Selectin/CD62E Products

Product Documents for Rat E-Selectin/CD62E ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat E-Selectin/CD62E ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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