Human Fas Ligand/TNFSF6 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91262

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

cell culture supernatant, serum, plasma (EDTA, citrate, heparin), amniotic fluid (50 uL)

Sensitivity

0.07 ng/mL

Assay Range

0.16 - 10.0 ng/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human Fas-L positive serum. There was no crossreactivity detected.

Description

The human sFas-L ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sFas-L.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 6.1%

Inter-Assay Precision (Precision between assays) CV% < 7.0%

Recovery for Human Fas Ligand/TNFSF6 ELISA Kit (Colorimetric)

Recovery

The recovery ranged from 82% to 98% with an overall mean recovery of 85%. Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Human Fas Ligand/TNFSF6 ELISA Kit (Colorimetric)

ELISA: Fas Ligand ELISA Kit 96 Tests [NBP1-91262]

Preparation and Storage

Formulation

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Fas Ligand/TNFSF6

Fas (APO-1, CD95) is a type I membrane protein that belongs to the TNF/nerve growth factor receptor family. Fas mediates apoptosis, the programmed cell death, when it is cross-linked with specific binding partners. The natural binding partner of Fas is its ligand, Fas-L, which is a 37 kDa type II-membrane protein that belongs to the TNF family which includes TNF, lymphotoxin, TNF-related apoptosis-inducing ligand (TRAIL), CD40 ligand, CD27 ligand, CD30 ligand, and OX40 ligand. Fas-L is predominantly expressed on activated T-cells and NK cells, thus Fas-L-mediated cell death is involved in the T or NK cell-mediated cytotoxicity, some pathologic tissue damages, and the regulation of lymphocyte homeostasis. A soluble form of Fas-L (sFas-L) is naturally produced by metalloproteinase- mediated processing. The soluble form resulting from this cleavage was shown to induce apoptosis in susceptible cells. Markedly elevated levels of sFas-L have been shown in TEN (Toxic Epidermal Necrolysis, Lyell's Syndrom) patients' sera. Liver dysfunction was shown to be paralleled by increased sFas-L levels as well as kidney damage. Fas-L is discussed to be involved in the pathogenesis of autoimmune diseases, especially the concentrations of sFas-L are remarkably higher in the sera and synovial fluids of patients with severe rheumatoid arthritis as compared to normal controls. Increased levels of soluble Fas-L in the serum of graft-versus-host disease patients make it a good marker for treatment of the disease.

Alternate Names

CD178, CD95L, FASLG, TNFSF6

Gene Symbol

FASLG

Additional Fas Ligand/TNFSF6 Products

Product Documents for Human Fas Ligand/TNFSF6 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Fas Ligand/TNFSF6 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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