Human Chitinase 3-like 1/YKL-40 ELISA Kit - Quantikine Best Seller
R&D Systems, part of Bio-Techne | Catalog # DC3L10
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL)
Sensitivity
8.15 pg/mL
Assay Range
62.5-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit
The Quantikine Human CHI3L1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure CHI3L1 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human CHI3L1 and antibodies raised against recombinant human CHI3L1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural CHI3L1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human CHI3L1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human CHI3L1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma/Urine - Samples from apparently healthy volunteers were evaluated for the
presence of human CHI3L1 in this assay. No medical histories were available for the donors
used in this study.
Cell Culture Supernates:
| Sample Type | Mean (ng/mL) | Range (ng/mL) | Standard Deviation (ng/mL) |
| Serum (n=36) | 36.0 | 15.9-93.5 | 20.6 |
| EDTA plasma (n=36) | 27.7 | 10.9-79.1 | 17.5 |
| Heparin plasma (n=36) | 26.8 | 10.7-72.1 | 15.7 |
| Urine (n=11) | 0.64 | 0.14-1.82 | 0.49 |
Cell Culture Supernates:
HepG2 human hepatocellular carcinoma cells were cultured in DMEM supplemented with
10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin
sulfate until confluent. An aliquot of the cell culture supernate was removed, assayed for
human CHI3L1, and measured 0.944 ng/mL.
MG-63 human osteosarcoma cells were cultured in DMEM supplemented with 10% fetal
bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate for
4 days. An aliquot of the cell culture supernate was removed, assayed for human CHI3L1, and
measured 3.04 ng/mL.
U-87 MG human glioblastoma/astrocytoma cells and SK-Mel-28 human malignant melanoma
cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2 mM L-glutamine,
100 U/mL penicillin, 1 mM sodium pyruvate, and 100 μg/mL streptomycin sulfate until
confluent. An aliquot of each cell culture supernate was removed and assayed for human
CHI3L1. U-87 MG cells measured 16.1 ng/mL, and the SK-Mel-28 cells measured 0.071 ng/mL.
HASMC human aortic smooth muscle cells were cultured in Medium 231 supplemented with
smooth muscle growth supplement until confluent. An aliquot of the cell culture supernate
was removed, assayed for human CHI3L1, and measured 0.623 ng/mL.
BUD-8 human fibroblasts were cultured in 90% MEM with non-essential amino acids
supplemented with 10% fetal bovine serum and 2 mM L-glutamine until confluent. An aliquot
of the cell culture supernate was removed, assayed for human CHI3L1, and measured
4.31 ng/mL.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 566 | 1031 | 2202 | 606 | 1019 | 2212 |
| Standard Deviation | 26.7 | 44.4 | 102.6 | 32.1 | 59.1 | 153.1 |
| CV% | 4.7 | 4.3 | 4.7 | 5.3 | 5.8 | 6.9 |
Recovery for Human Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit
The recovery of CHI3L1 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 97 | 93-100 |
| Urine (n=4) | 105 | 90-115 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of CHI3L1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit
Human Chitinase 3-like 1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Chitinase 3-like 1/YKL-40
Alternate Names
CHI3L1, Chitinase 3 like 1, HCgp39, YKL-40
Gene Symbol
CHI3L1
Additional Chitinase 3-like 1/YKL-40 Products
- All Products for Chitinase 3-like 1/YKL-40
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- Chitinase 3-like 1/YKL-40 Luminex Assays
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- Chitinase 3-like 1/YKL-40 Proteins and Enzymes
- Chitinase 3-like 1/YKL-40 Simple Plex
Product Documents for Human Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit
Product Specific Notices for Human Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.