Human Cyr61/CCN1 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DCYR10
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Saliva (50 µL), Human Milk (10 µL)
Sensitivity
3.8 pg/mL
Assay Range
39.1-2500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Saliva, Human Milk)
Product Summary for Human Cyr61/CCN1 Quantikine ELISA Kit
The Quantikine Human Cyr61/CCN1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Cyr61 in cell culture supernates, serum, plasma, saliva, and human milk. It contains CHO cell-expressed recombinant human Cyr61 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Cyr61 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Cyr61.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Cyr61
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Human Milk, Saliva, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 256 | 721 | 1442 | 249 | 712 | 1445 |
| Standard Deviation | 5.94 | 14.2 | 33.5 | 15.9 | 35.0 | 73.3 |
| CV% | 2.3 | 2.0 | 2.3 | 6.4 | 4.9 | 5.1 |
Recovery for Human Cyr61/CCN1 Quantikine ELISA Kit
The recovery of Cyr61 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 107 | 98-117 |
| EDTA Plasma (n=4) | 98 | 91-104 |
| Serum (n=4) | 101 | 95-113 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Cyr61 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Cyr61/CCN1 Quantikine ELISA Kit
Human Cyr61/CCN1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Cyr61/CCN1
Cyr61, also known as IGFBP-10 and CCN1, is a 50 kDa matricellular glycoprotein that regulates the growth and adhesion of vascular endothelial cells, fibroblasts, and monocytes. Cyr61 induces VEGF upregulation, angiogenesis, and tumorigenesis through interactions with integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1. Cyr61 is cleaved by plasmin within its VWF domain which generates an N-terminal fragment that is not associated with the matrix but retains the ability to induce endothelial cell migration.
Long Name
Cysteine-Rich, Angiogenic Inducer 61
Alternate Names
CCN1, GIG1, IGFBP-10
Gene Symbol
CCN1
Additional Cyr61/CCN1 Products
Product Documents for Human Cyr61/CCN1 Quantikine ELISA Kit
Product Specific Notices for Human Cyr61/CCN1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.