Human FGF acidic/FGF1 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DFA00B
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Urine (50 µL)
Sensitivity
13.9 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human FGF acidic/FGF1 Quantikine ELISA Kit
The Quantikine Human FGF acidic Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FGF acidic in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human FGF acidic and has been shown to accurately quantitate the recombinant factor.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human FGF acidic
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 208 | 485 | 766 | 204 | 477 | 761 |
| Standard Deviation | 7.6 | 21.8 | 22.9 | 15.0 | 29.3 | 48.7 |
| CV% | 3.7 | 4.5 | 3.0 | 7.4 | 6.1 | 6.4 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 179 | 441 | 749 | 178 | 433 | 713 |
| Standard Deviation | 8.3 | 31.8 | 17.6 | 15.0 | 36.3 | 61.0 |
| CV% | 4.6 | 7.2 | 2.3 | 8.4 | 8.4 | 8.6 |
Recovery for Human FGF acidic/FGF1 Quantikine ELISA Kit
The recovery of FGF acidic spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 105 | 93-113 |
| EDTA Plasma (n=4) | 102 | 93-109 |
| Heparin Plasma (n=4) | 97 | 92-110 |
| Serum (n=4) | 98 | 89-108 |
| Urine (n=4) | 99 | 89-108 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of FGF acidic were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human FGF acidic/FGF1 Quantikine ELISA Kit
Human FGF acidic ELISA Cell Culture Supernate/Urine Standard Curve
Human FGF acidic ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: FGF acidic/FGF1
Long Name
Fibroblast Growth Factor acidic
Alternate Names
alpha-ECGF, beta-ECGF, FGF acidic, FGF-1, HBGF-1
Gene Symbol
FGF1
Additional FGF acidic/FGF1 Products
Product Documents for Human FGF acidic/FGF1 Quantikine ELISA Kit
Product Specific Notices for Human FGF acidic/FGF1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.