Human FGF basic/FGF2/bFGF Quantikine HS ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # HSFB00D
Key Product Details
Assay Length
5.5 hours
Sample Type & Volume Required Per Well
Serum (100 µL), EDTA Plasma (100 µL), Citrate Plasma (100 µL), Urine (100 µL)
Sensitivity
0.07 pg/mL
Assay Range
0.313-20 pg/mL (Serum, EDTA Plasma, Citrate Plasma, Urine)
Product Summary for Human FGF basic/FGF2/bFGF Quantikine HS ELISA Kit
The Quantikine HS Human FGF basic Immunoassay kit is a 5.5 hour solid phase ELISA designed to measure FGF basic levels in serum, plasma, and urine. It contains E. coli-expressed recombinant human FGF basic and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human FGF basic accurately. Results obtained using natural human FGF basic showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human FGF basic.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450 or 490nm (TMB or AP Substrate)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human FGF basic
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Citrate Plasma, EDTA Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 1.31 | 5.32 | 11.4 | 1.51 | 5.71 | 11.1 |
| Standard Deviation | 0.10 | 0.23 | 0.40 | 0.12 | 0.27 | 0.91 |
| CV% | 7.6 | 4.3 | 3.5 | 7.9 | 4.7 | 8.2 |
Recovery for Human FGF basic/FGF2/bFGF Quantikine HS ELISA Kit
The recovery of FGF basic spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Citrate Plasma (n=4) | 97 | 83-118 |
| EDTA Plasma (n=4) | 99 | 85-109 |
| Serum (n=4) | 100 | 88-116 |
| Urine (n=4) | 109 | 100-120 |
Linearity
To assess the linearity of the assay, the following biological samples spiked with high concentrations of FGF basic were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Human FGF basic/FGF2/bFGF Quantikine HS ELISA Kit
Human FGF basic ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: FGF basic/FGF2/bFGF
Long Name
Fibroblast Growth Factor basic
Alternate Names
bFGF, FGF-2, FGF2, HBGF-2, Prostatropin
Gene Symbol
FGF2
Additional FGF basic/FGF2/bFGF Products
Product Documents for Human FGF basic/FGF2/bFGF Quantikine HS ELISA Kit
Product Specific Notices for Human FGF basic/FGF2/bFGF Quantikine HS ELISA Kit
For research use only
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