Human IL-13 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # D1300B
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
Sensitivity
57.4 pg/mL
Assay Range
125-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human IL-13 Quantikine ELISA Kit
The Quantikine Human IL-13 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-13 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-13 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-13 accurately. Results obtained using natural human IL-13 showed dose response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-13.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-13
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 360 | 1031 | 2002 | 319 | 932 | 1895 |
| Standard Deviation | 12.9 | 27.5 | 43.2 | 22.2 | 52.6 | 108 |
| CV% | 3.6 | 2.7 | 2.2 | 7.0 | 5.6 | 5.7 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 352 | 960 | 1919 | 365 | 1087 | 2199 |
| Standard Deviation | 11.1 | 21.3 | 33.3 | 25.8 | 63.1 | 105 |
| CV% | 3.2 | 2.2 | 1.7 | 7.1 | 5.8 | 4.8 |
Recovery for Human IL-13 Quantikine ELISA Kit
The recovery of IL-13 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 108 | 100-120 |
| EDTA Plasma (n=4) | 106 | 98-120 |
| Heparin Plasma (n=4) | 104 | 94-120 |
| Serum (n=4) | 102 | 95-113 |
Linearity
To assess linearity of the assay, samples were spiked with high concentrations of IL-13 and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IL-13 Quantikine ELISA Kit
Human IL-13 ELISA Calibrator Diluent RD5R Standard Curve
Human IL-13 ELISA Calibrator Diluent RD6-64 Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-13
Long Name
Interleukin 13
Alternate Names
IL13, NC30, P600
Gene Symbol
IL13
Additional IL-13 Products
Product Documents for Human IL-13 Quantikine ELISA Kit
Product Specific Notices for Human IL-13 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.