Human Progranulin Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DPGRN0
Not Available in Europe
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (25 µL), Human Milk (10 µL)
Sensitivity
0.54 ng/mL
Assay Range
1.56-100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Product Summary for Human Progranulin Quantikine ELISA Kit
The Quantikine Human Progranulin immunoassay is a 4.5 hour solid phase ELISA designed to measure Progranulin in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human Progranulin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural Progranulin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Progranulin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Progranulin. Trypsin and Elastase cleaved Progranulin show reduced recognition in this assay, demonstrating specificity for Progranulin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Saliva
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 13.5 | 32.7 | 64.8 | 14.4 | 33.1 | 64.4 |
| Standard Deviation | 0.7 | 1.9 | 4.1 | 1.0 | 2.3 | 4.4 |
| CV% | 5.2 | 5.8 | 6.3 | 6.9 | 6.9 | 6.8 |
EDTA Plasma, Heparin Plasma, Human Milk, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 15.5 | 36.6 | 70.8 | 15.7 | 35.8 | 69.1 |
| Standard Deviation | 0.9 | 1.7 | 3.1 | 1.3 | 2.9 | 5.1 |
| CV% | 5.8 | 4.6 | 4.4 | 8.3 | 8.1 | 7.4 |
Recovery for Human Progranulin Quantikine ELISA Kit
The recovery of Progranulin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 107 | 103-112 |
| EDTA Plasma (n=4) | 102 | 95-110 |
| Heparin Plasma (n=4) | 100 | 92-108 |
| Serum (n=4) | 104 | 90-115 |
| Urine (n=4) | 109 | 99-116 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Progranulin were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Progranulin Quantikine ELISA Kit
Human Progranulin/PGRN ELISA Serum/Plasma/Urine Human Milk Standard Curve
Human Progranulin/PGRN ELISA Cell Culture Supernate/Saliva Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Progranulin/PGRN
Alternate Names
Acrogranin, GEP, GP88, GRN, PCDGF, PEPI, PGRN, Proepithelin
Gene Symbol
GRN
Additional Progranulin/PGRN Products
Product Documents for Human Progranulin Quantikine ELISA Kit
Product Specific Notices for Human Progranulin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.