Human ST2/IL-33R Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DST200
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
13.5 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human ST2/IL-33R Quantikine ELISA Kit
The Quantikine Human ST2/IL-33 R Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human ST2 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human ST2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ST2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring ST2.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human ST2 as well as free ST2 and IL-33 complexed ST2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence
of human ST2 in this assay. No medical histories were available for the donors used in this
study.
Cell Culture Supernates: BJAB human Burkitt's lymphoma cells (1 x 105 cells/mL) were seeded in DMEM supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human ST2, and measured 126 pg/mL.
| Sample Type | Mean (ng/mL) | Range (ng/mL) | Standard Devitation (ng/mL) |
| Serum (n=35) | 13.0 | 6.74-20.4 | 4.15 |
| Heparin plasma (n=35) | 12.3 | 5.72-19.8 | 4.10 |
| EDTA plasma (n=35) | 12.2 | 4.90-19.9 | 4.08 |
Cell Culture Supernates: BJAB human Burkitt's lymphoma cells (1 x 105 cells/mL) were seeded in DMEM supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human ST2, and measured 126 pg/mL.
BUD-8 human fibroblast cells were seeded in MEM NEAA 90% supplemented with 10% fetal
bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate
until confluent. An aliquot of the cell culture supernate was removed, assayed for human ST2,
and measured 273 pg/mL.
U-87 MG human glioblastoma/astrocytoma cells (2.1 x 105
cells/mL) were seeded in MEM
supplemented with 10% fetal bovine serum, 1% sodium pyruvate, and 2 mM L-glutamine until
confluent. An aliquot of the cell culture supernate was removed, assayed for human ST2, and
measured 63.9 pg/mL.
HUVEC human umbilical vein endothelial cells (1 x 104
cells/mL) were seeded in EGM-2 media
until confluent. An aliquot of the cell culture supernate was removed, assayed for human ST2,
and measured 29,920 pg/mL.
HUT-78 human cutaneous T cell lymphoma cells (1 x 104
cells/mL) were seeded in RPMI
supplemented with 10% fetal bovine serum, 5 μM beta-mercaptoethanol, and 10 ng/mL
recombinant human IL-2 until confluent. An aliquot of the cell culture supernate was removed,
assayed for human ST2, and measured 54.9 pg/mL.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 273 | 628 | 1027 | 262 | 642 | 1064 |
| Standard Deviation | 15.2 | 27.5 | 46.3 | 18.7 | 34.7 | 67.2 |
| CV% | 5.6 | 4.4 | 4.5 | 7.1 | 5.4 | 6.3 |
Recovery for Human ST2/IL-33R Quantikine ELISA Kit
The recovery of ST2 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 97 | 89-106 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of ST2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human ST2/IL-33R Quantikine ELISA Kit
Human ST2/IL-1 R4 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: ST2/IL-33R
Long Name
Interleukin 33 Receptor
Alternate Names
DER4, Fit-1, IL-1 R4, IL-1R4, IL-1RL1, IL-33 R, IL-33R, IL1R4, IL33R, Ly84, ST2L, ST2V, T1
Gene Symbol
IL1RL1
Additional ST2/IL-33R Products
Product Documents for Human ST2/IL-33R Quantikine ELISA Kit
Product Specific Notices for Human ST2/IL-33R Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.