Human TRAIL/TNFSF10 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DTRL00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (25 µL)
Sensitivity
7.87 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Product Summary for Human TRAIL/TNFSF10 Quantikine ELISA Kit
The Quantikine Human TRAIL Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human TRAIL in cell culture supernates, serum, plasma, cell lysates, and saliva. It contains NS0-expressed recombinant human TRAIL and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TRAIL showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TRAIL
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human TRAIL
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, Saliva
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 95.0 | 193 | 391 | 96.0 | 191 | 388 |
| Standard Deviation | 4.26 | 8.81 | 24.3 | 8.30 | 14.8 | 22.2 |
| CV% | 4.5 | 4.6 | 6.2 | 8.6 | 7.7 | 5.7 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 102 | 203 | 408 | 102 | 202 | 407 |
| Standard Deviation | 3.45 | 5.73 | 23.0 | 7.59 | 12.2 | 17.8 |
| CV% | 3.4 | 2.8 | 5.6 | 7.5 | 6.1 | 4.4 |
Recovery for Human TRAIL/TNFSF10 Quantikine ELISA Kit
The recovery of TRAIL spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 101 | 92-105 |
| EDTA Plasma (n=4) | 103 | 96-112 |
| Heparin Plasma (n=4) | 103 | 98-115 |
| Serum (n=4) | 96 | 91-102 |
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of TRAIL were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human TRAIL/TNFSF10 Quantikine ELISA Kit
Human TRAIL/TNFSF10 ELISA Cell Culture Supernate/Cell Lysate/Saliva Standard Curve
Human TRAIL/TNFSF10 ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TRAIL/TNFSF10
Long Name
TNF-related Apoptosis-inducing Ligand
Alternate Names
CD253, TNFSF10
Gene Symbol
TNFSF10
Additional TRAIL/TNFSF10 Products
Product Documents for Human TRAIL/TNFSF10 Quantikine ELISA Kit
Product Specific Notices for Human TRAIL/TNFSF10 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.