Mouse IL-12 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92658

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

cell culture supernatant, serum (50 uL )

Sensitivity

10.0 pg/mL

Assay Range

15.6 - 1,000 pg/mL

View all IL-12 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

The unspiked serum was used as blank in these experiments. The recovery ranged from 95% to 118% with an overall mean recovery of 106%

Description

This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 8.3 %

Inter-Assay Precision (Precision between assays) CV% < 11.0 %

Recovery for Mouse IL-12 ELISA Kit (Colorimetric)

Recovery

The unspiked serum was used as blank in these experiments. The recovery ranged from 95% to 118% with an overall mean recovery of 106%

Scientific Data Images for Mouse IL-12 ELISA Kit (Colorimetric)

ELISA: Mouse IL-12 ELISA Kit (Colorimetric) [NBP1-92658]

ELISA: Mouse IL-12 ELISA Kit (Colorimetric) [NBP1-92658]

ELISA: Mouse IL-12 ELISA Kit (Colorimetric) [NBP1-92658] - IL-12 ELISA Kit (Colorimetric) [NBP1-92658] - Representative standard curve for mouse IL-12 ELISA. Mouse IL- 12 was diluted in serial 2-fold steps in sample diluent. Do not use this standard curve to derive test results. A standard curve must be run for each group ofmicrowell strips assayed.

Kit Contents for Mouse IL-12 ELISA Kit (Colorimetric)

  • Biotin-Conjugate anti-mouse IL-12 p70 monoclonal antibody Streptavidin-HRP
  • Blue-Dye
  • Green-Dye
  • Mouse IL-12 p70 Standard lyophilized, 2ng/mL upon reconstitution
  • Red-Dye

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-12

Interleukin-12 is produced primarily by stimulated macrophages. IL-12 has been shown to be a proinflammatory cytokine produced by phagocytic cells, B cells, and other antigen - presenting cells that modulate adaptive immune responses by favoring the generation of T-helper type 1 cells. IL-12 exerts a variety of biological effects on T and natural killer cells. Apart from promotion of Th1 development and its ability to promote cytolytic activity it mediates some of its physiological activities by acting as a potent inducer of IFN-gamma production and the stimulation of other cytokines from peripheral blood T and NK cells. IFN-gamma then enhances the ability of the phagocytic cells to produce IL-12 and other proinflammatory cytokines. Thus, IL-12 induced IFN- gamma acts in a positive feedback loop that represents an important amplifying mechanism in the inflammatory response to infections. Its role in directing development of a Th1 type immune response from naive T cells demonstrates its critical role in regulation of the immune response and strongly suggests its potential usefulness in cancer therapy.

Long Name

Interleukin 12

Alternate Names

CLMF1, IL12, IL12A, NKSF1

Gene Symbol

IL12A

Additional IL-12 Products

Product Documents for Mouse IL-12 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse IL-12 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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