Mouse IL-12 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92677

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-92677
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum (50 uL)

Sensitivity

4.0 pg/mL

Assay Range

31.3 - 2,000 pg/mL

View all IL-12 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

The interference of circulating factors of the immune systemwas evaluated by spiking these proteins at physiologically relevant concentrations into a mouse IL-12 positive serum. There was no crossreactivity detected.

Description

The mouse IL-12 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-12.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.0%

Inter-Assay Precision (Precision between assays) CV% < 10%

Recovery for Mouse IL-12 ELISA Kit (Colorimetric)

Recovery

93%

Scientific Data Images for Mouse IL-12 ELISA Kit (Colorimetric)

ELISA: Mouse IL-12 ELISA Kit (Colorimetric) [NBP1-92677]

ELISA: Mouse IL-12 ELISA Kit (Colorimetric) [NBP1-92677]

ELISA: IL-12 ELISA Kit [Biotin] [NBP1-92677] - Standard curve

Kit Contents for Mouse IL-12 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-12
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-mouse IL-12 monoclonal antibody
  • Mouse IL-12 Standard lyophilized, 4 ng/ml upon reconstitution
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-12

Interleukin-12 is produced primarily by stimulated macrophages. IL-12 has been shown to be a proinflammatory cytokine produced by phagocytic cells, B cells, and other antigen - presenting cells that modulate adaptive immune responses by favoring the generation of T-helper type 1 cells. IL-12 exerts a variety of biological effects on T and natural killer cells. Apart from promotion of Th1 development and its ability to promote cytolytic activity it mediates some of its physiological activities by acting as a potent inducer of IFN-gamma production and the stimulation of other cytokines from peripheral blood T and NK cells. IFN-gamma then enhances the ability of the phagocytic cells to produce IL-12 and other proinflammatory cytokines. Thus, IL-12 induced IFN- gamma acts in a positive feedback loop that represents an important amplifying mechanism in the inflammatory response to infections. Its role in directing development of a Th1 type immune response from naive T cells demonstrates its critical role in regulation of the immune response and strongly suggests its potential usefulness in cancer therapy.

Long Name

Interleukin 12

Alternate Names

CLMF1, IL12, IL12A, NKSF1

Gene Symbol

IL12A

Additional IL-12 Products

Product Documents for Mouse IL-12 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse IL-12 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.