Human IL-12/IL-23 p40 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82421

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (citrate, heparin), buffered solution (50 uL )

Sensitivity

< 10 pg/mL

Assay Range

31.3 - 2000 pg/mL

View all IL-12/IL-23 p40 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The assay detects both natural and recombinant human IL12B. The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations. There was no crossreactivity observed with IL-1 beta, IL- 2, IL-4, IL-5, IL-6, IL-8, IL-10, IFN gamma, TNF alpha and TNF beta. BMS2013 also detects IL/23and IL12p70.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.7%

Inter-Assay Precision (Precision between assays) CV% < 6.4%

Recovery for Human IL-12/IL-23 p40 ELISA Kit (Colorimetric)

Recovery

The recovery ranged from 65% to 75% with an overall mean recovery of 71%.

Scientific Data Images for Human IL-12/IL-23 p40 ELISA Kit (Colorimetric)

ELISA: Human IL-12/IL-23 p40 ELISA Kit (Colorimetric) [NBP1-82421]

ELISA: Human IL-12/IL-23 p40 ELISA Kit (Colorimetric) [NBP1-82421]

ELISA: Human IL12B ELISA Kit (Colorimetric) [NBP1-82421] - Representative standard curve for human IL12B ELISA

Kit Contents for Human IL-12/IL-23 p40 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human IL-12/IL-23 p40
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-human IL-12/IL-23 p40 monoclonal antibody Streptavidin-HRP
  • Blue-Dye
  • Green-Dye
  • Human IL-12/IL-23 p40 Standard lyophilized, 4ng/mL upon reconstitution
  • Red-Dye
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-12/IL-23 p40

Biologically active IL-12 is a disulfide-linked heterodimeric 70-kDa cytokine composed of a 35-kDa (p35) and a 40-kDa (p40) subunit. P35, a member of the IL-6 superfamily, is secreted in response to IFN gamma and agonists of TLR3, 4, or 7. However, p35 expression has been shown to be inhibited by Th2 cytokines and expressed at much lower levels than p40. Expression of p40 is regulated independently of p35. Moreover, p40 has been shown to be secreted as either a monomer or homodimer. Although each subunit alone does not possess IL-12 bioactivity, the p40 homodimer can bind to the IL-12 receptor and act as an antagonist to IL-12 p70. The receptor for this cytokine is composed of two subunits, IL-12R beta 1 and IL-12R beta 2, the latter of which is the signaling component of the receptor.

Long Name

Interleukin 12/Interleukin 23 p40

Alternate Names

CLMF2, IL-12B, IL12 p40, IL12B, NKSF2

Gene Symbol

IL12B

Additional IL-12/IL-23 p40 Products

Product Documents for Human IL-12/IL-23 p40 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human IL-12/IL-23 p40 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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