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Mouse Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18772

Novus Biologicals, part of Bio-Techne
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NBP3-18772

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

9.38 pg/mL

Assay Range

15.63-1000 pg/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse Lymphotoxin-alpha/TNF-beta in samples. No significant cross-reactivity or interference between Mouse Lymphotoxin-alpha/TNF-beta and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 5.82

Inter-Assay Precision (Precision between assays) %CV < 5.73

Recovery for Mouse Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric)

Recovery

90-108%

Linearity

Mouse Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric)

Scientific Data Images for Mouse Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric)

Mouse Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric)

ELISA: Mouse Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric) [NBP3-18772] -

ELISA: Mouse Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric) [NBP3-18772] - Standard Curve Reference

Kit Contents for Mouse Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Lymphotoxin-alpha/TNF-beta

TNF-beta (tumor necrosis factor beta, lymphotoxin alpha, TNFSF1, TNF ligand superfamily member 1) a member of the TNF ligand superfamily and a potent lymphoid factor, is mainly produced by activated T and B lymphocytes and leukocytes. TNF-beta is also produced by fibroblasts, astrocytes, myeloma cells, endothelial cells and epithelial cells. TNF-beta mediates inflammatory and immune responses, has cytotoxic activity on tumor cells and other target cells and shares many of the same bioactivities as TNF alpha, including regulation of cell proliferation, differentiation and apoptosis. TNF-beta is not species-specific and human TNF-beta is active on murine cells with a slightly reduced specific activity.

Long Name

Tumor Necrosis Factor beta

Alternate Names

LTA, Lymphotoxin alpha, Lymphotoxinalpha, TNF-beta, tnfb, TNFSF1, TNFSF1B

Gene Symbol

LTA

Additional Lymphotoxin-alpha/TNF-beta Products

Product Documents for Mouse Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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