Mouse Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MC3L10
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
16 pg/mL
Assay Range
62.5-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit
The Quantikine Mouse CHI3L1 immunoassay is a 4.5 hour solid-phase ELISA designed to measure CHI3L1 in cell culture supernates, mouse serum, and plasma. It contains NS0-expressed recombinant mouse CHI3L1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse CHI3L1. Results obtained using natural mouse CHI3L1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse CHI3L1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse CHI3L1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 187 | 480 | 1533 | 205 | 511 | 1530 |
| Standard Deviation | 10.4 | 34.5 | 67.7 | 18.7 | 37.1 | 117 |
| CV% | 5.6 | 7.2 | 4.4 | 9.1 | 7.3 | 7.6 |
Recovery for Mouse Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit
The recovery of mouse CHI3L1 spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 99 | 88-107 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse CHI3L1 in each matrix were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Mouse Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit
Mouse Chitinase 3-like 1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Chitinase 3-like 1/YKL-40
Alternate Names
CHI3L1, Chitinase 3 like 1, HCgp39, YKL-40
Gene Symbol
CHI3L1
Additional Chitinase 3-like 1/YKL-40 Products
- All Products for Chitinase 3-like 1/YKL-40
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- Chitinase 3-like 1/YKL-40 ELISA Kits
- Chitinase 3-like 1/YKL-40 Luminex Assays
- Chitinase 3-like 1/YKL-40 Lysates
- Chitinase 3-like 1/YKL-40 Primary Antibodies
- Chitinase 3-like 1/YKL-40 Proteins and Enzymes
- Chitinase 3-like 1/YKL-40 Simple Plex
Product Documents for Mouse Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit
Product Specific Notices for Mouse Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.