Mouse IL-12 p40 Allele-specific Quantikine ELISA Kt
R&D Systems, part of Bio-Techne | Catalog # M1240
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL)
Sensitivity
4 pg/mL
Assay Range
11.7-750 pg/mL (Cell Culture Supernates, Serum)
Product Summary for Mouse IL-12 p40 Allele-specific Quantikine ELISA Kt
The Quantikine Mouse IL-12/IL-23 p40 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse p40 subunit in IL-12 p70, IL-23, p40 homodimer, and p40 monomer in cell culture supernates and mouse serum. It contains recombinant mouse IL-12 p40 homodimer and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse IL-12/IL-23 p40. Results obtained using natural mouse IL-12/IL-23 p40 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-12/IL-23 p40.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse IL-12/IL-23 p40
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 42.2 | 161 | 588 | 41.9 | 151 | 581 |
| Standard Deviation | 0.81 | 1.7 | 9.6 | 4.1 | 10.3 | 24.0 |
| CV% | 1.9 | 1.1 | 1.6 | 9.8 | 6.8 | 4.1 |
Recovery for Mouse IL-12 p40 Allele-specific Quantikine ELISA Kt
The recovery of mouse IL-12/IL-23 p40 spiked to levels in samples throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=11) | 100 | 81-116 |
| Serum (n=7) | 104 | 85-115 |
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse IL-12/IL-23 p40 in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse IL-12 p40 Allele-specific Quantikine ELISA Kt
Mouse IL-12/IL-23 p40 Allele-specific ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-12/IL-23 p40
Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.
Long Name
Interleukin 12/Interleukin 23 p40
Alternate Names
CLMF2, IL-12B, IL12 p40, IL12B, NKSF2
Entrez Gene IDs
Gene Symbol
IL12B
Additional IL-12/IL-23 p40 Products
Product Documents for Mouse IL-12 p40 Allele-specific Quantikine ELISA Kt
Product Specific Notices for Mouse IL-12 p40 Allele-specific Quantikine ELISA Kt
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.