Mouse ST2/IL-33R Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MST200
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.059 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse ST2/IL-33R Quantikine ELISA Kit
The Quantikine Mouse ST2/IL-33 R Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse ST2 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant mouse ST2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse ST2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse ST2.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse ST2
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 0.465 | 1.34 | 3.81 | 0.512 | 1.43 | 3.94 |
| Standard Deviation | 0.019 | 0.042 | 0.106 | 0.047 | 0.082 | 0.138 |
| CV% | 4.1 | 3.1 | 2.8 | 9.2 | 5.7 | 3.5 |
Recovery for Mouse ST2/IL-33R Quantikine ELISA Kit
The recovery of mouse ST2 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 98 | 81-111 |
| EDTA Plasma (n=4) | 104 | 92-118 |
| Heparin Plasma (n=4) | 94 | 86-103 |
| Serum (n=4) | 103 | 89-113 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse ST2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse ST2/IL-33R Quantikine ELISA Kit
Mouse ST2/IL-1 R4 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: ST2/IL-33R
Long Name
Interleukin 33 Receptor
Alternate Names
DER4, Fit-1, IL-1 R4, IL-1R4, IL-1RL1, IL-33 R, IL-33R, IL1R4, IL33R, Ly84, ST2L, ST2V, T1
Gene Symbol
IL1RL1
Additional ST2/IL-33R Products
Product Documents for Mouse ST2/IL-33R Quantikine ELISA Kit
Product Specific Notices for Mouse ST2/IL-33R Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.