Mouse Tie-2 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MTE200
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)
Sensitivity
40.2 pg/mL
Assay Range
125-8000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse Tie-2 Quantikine ELISA Kit
The Quantikine Mouse Tie-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse Tie-2 in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant mouse Tie-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse Tie-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse Tie-2.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse Tie-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 21 | 21 | 21 |
| Mean (pg/mL) | 395 | 1149 | 3470 | 434 | 1187 | 3183 |
| Standard Deviation | 26.1 | 69.1 | 220 | 34.5 | 77.6 | 147 |
| CV% | 6.6 | 6.0 | 6.3 | 7.9 | 6.5 | 4.6 |
Recovery for Mouse Tie-2 Quantikine ELISA Kit
The recovery of mouse Tie-2 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 106 | 98-119 |
| EDTA Plasma (n=5) | 107 | 95-120 |
| Heparin Plasma (n=4) | 102 | 87-113 |
| Serum (n=4) | 103 | 93-116 |
Linearity
To assess the linearity of the assay, samples containing or spiked with recombinant mouse Tie-2 in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse Tie-2 Quantikine ELISA Kit
Mouse Tie-2 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Tie-2
Long Name
Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
Alternate Names
CD202b, TEK, Tie2
Entrez Gene IDs
Gene Symbol
TEK
Additional Tie-2 Products
Product Documents for Mouse Tie-2 Quantikine ELISA Kit
Product Specific Notices for Mouse Tie-2 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.