Rat P-Selectin/CD62P ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76614

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.188ng/mL

Assay Range

0.313 - 20ng/mL

View all P-Selectin/CD62P ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat SELP in samples. No significant cross-reactivity or interference between Rat SELP and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 6.64

Inter-Assay Precision (Precision between assays) CV% < 5.64

Recovery for Rat P-Selectin/CD62P ELISA Kit (Colorimetric)

Recovery

85-100%

Linearity

Rat P-Selectin/CD62P ELISA Kit (Colorimetric)

Scientific Data Images for Rat P-Selectin/CD62P ELISA Kit (Colorimetric)

Rat P-Selectin/CD62P ELISA Kit (Colorimetric)

ELISA: Rat P-Selectin/CD62P ELISA Kit (Colorimetric) [NBP2-76614] -

ELISA: Rat P-Selectin/CD62P ELISA Kit (Colorimetric) [NBP2-76614] - Standard Curve Reference

Kit Contents for Rat P-Selectin/CD62P ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: P-Selectin/CD62P

CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.

Alternate Names

CD62P, GMP140, GRMP, PADGEM, PSEL, SELP

Gene Symbol

SELP

Additional P-Selectin/CD62P Products

Product Documents for Rat P-Selectin/CD62P ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat P-Selectin/CD62P ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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