Rat Sonic Hedgehog/Shh ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68123

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

View all Sonic Hedgehog/Shh ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat SHH in samples. No significant cross-reactivity or interference between Rat SHH and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.75%

Inter-Assay Precision (Precision between assays) CV% < 5.26%

Recovery for Rat Sonic Hedgehog/Shh ELISA Kit (Colorimetric)

Recovery

86-110%

Linearity

Rat Sonic Hedgehog/Shh ELISA Kit (Colorimetric)

Scientific Data Images for Rat Sonic Hedgehog/Shh ELISA Kit (Colorimetric)

Rat Sonic Hedgehog/Shh ELISA Kit (Colorimetric)

ELISA: Rat Sonic Hedgehog/Shh ELISA Kit (Colorimetric) [NBP2-68123] -

ELISA: Rat Sonic Hedgehog/Shh ELISA Kit (Colorimetric) [NBP2-68123] - Standard Curve Reference

Kit Contents for Rat Sonic Hedgehog/Shh ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Sonic Hedgehog/Shh

Sonic Hedgehog Protein (SHH, VHH-1) belongs to hedgehog protein family which includes Indian Hh, and Desert Hh. Hh family is involved in the cell fate and patterning during embryonic development, homeostasis, and adult tissue renewal (1). Similar to other member in the family, SHH binds to the patched (PTC) cell surface receptor, releasing the signal transducer Smoothened (Smo) to transmit the Hh signal into the cell and activate transcription of the target gene (2). Precursor SHH is autocatlytically cleaved into two subunits, N-terminal and C-terminal products. Soluble N-terminal product is involved in the signaling activity, while C-product displays an autoproteolysis activity on the precursor and a cholesterol transferase activity on the N-terminal product. C-terminal product attaches a cholesterol moiety to the N-terminal product, preventing N-terminal product diffusion within the developing embryo (3). A defect in SHH has been linked in holoprosencephaly type 3 (HPE3), in which developing forebrain fails to separate into right and left hemispheres, and ocular coloboma (4).

Alternate Names

HHG1, HLP3, HPE3, MCOPCB5, Shh, ShhNC, SMMCI, TPTPS

Gene Symbol

SHH

Additional Sonic Hedgehog/Shh Products

Product Documents for Rat Sonic Hedgehog/Shh ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Sonic Hedgehog/Shh ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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