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Recombinant Human Apolipoprotein A5 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22689

Novus Biologicals, part of Bio-Techne
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NBP2-22689

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 24-366 of Human Apolipoprotein A5

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSRKGFWDY FSQTSGDKGR VEQIHQQKMA REPATLKDSL EQDLNNMNKF LEKLRPLSGS EAPRLPQDPV GMRRQLQEEL EEVKARLQPY MAEAHELVGW NLEGLRQQLK PYTMDLMEQV ALRVQELQEQ LRVVGEDTKA QLLGGVDEAW ALLQGLQSRV VHHTGRFKEL FHPYAESLVS GIGRHVQELH RSVAPHAPAS PARLSRCVQV LSRKLTLKAK ALHARIQQNL DQLREELSRA FAGTGTEEGA GPDPQMLSEE VRQRLQAFRQ DTYLQIAAFT RAIDQETEEV QQQLAPPPPG HSAFAPEFQQ TDSGKVLSKL QARLDDLWED ITHSLHDQGH SHLGDP

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

41.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Apolipoprotein A5 His Protein

SDS-PAGE: Recombinant Human Apolipoprotein A5 His Protein [NBP2-22689]

SDS-PAGE: Recombinant Human Apolipoprotein A5 His Protein [NBP2-22689]

SDS-Page: Recombinant Human Apolipoprotein A5 Protein [NBP2-22689]

Formulation, Preparation and Storage

NBP2-22689
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.4M UREA
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Apolipoprotein A5

APOA5 is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified. Recombinant human APOA5 protein, fused to His-tag at N-terminus, was expressed in E.coli .

Alternate Names

APOAV, apo-AV, APOA-V, Apolipoprotein A5, apolipoprotein A-V, FLJ97995, MGC126836, RAP3MGC126838, Regeneration-associated protein 3

Gene Symbol

APOA5

Additional Apolipoprotein A5 Products

Product Documents for Recombinant Human Apolipoprotein A5 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human Apolipoprotein A5 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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