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ART5 Recombinant Protein Antigen

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48808PEP

Novus Biologicals, part of Bio-Techne
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NBP2-48808PEP

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Antibody Competition

Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human ART5.

Source: E. coli

Amino Acid Sequence: LQLLRGSGGCSRGPGEVVFRGVGSLRFEPKRLGDSVRLGQFASSSLDKAVAHRFGNATLFSLTTCFGAPIQAFSV

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10 - 100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-48808.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

For further blocking peptide related information and a protocol, click here.

Protein / Peptide Type

Recombinant Protein Antigen

Formulation, Preparation and Storage

NBP2-48808PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: ART5

ART5 belongs to the mammalian mono-ADP-ribosyltransferase (ART) family, named for the enzymatic reaction that some of its members - including ART5 - catalyze: the transfer of a single ADP-ribose residue from NAD+ to arginine in the extracellular acceptor protein. Unlike other ARTs, ART5 is thought to be secreted rather than membrane-bound. The effects of the reversible post-translational modification catalyzed by these ectoenzymes are currently under investigation.

Alternate Names

ADP-ribosyltransferase 5, EC 2.4.2.31, ecto-ADP-ribosyltransferase 5, MGC22848, Mono(ADP-ribosyl)transferase 5, NAD(P)(+)--arginine ADP-ribosyltransferase 5

Gene Symbol

ART5

Additional ART5 Products

Product Documents for ART5 Recombinant Protein Antigen

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ART5 Recombinant Protein Antigen

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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