Recombinant Human CCL3/MIP-1 alpha Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35011
Key Product Details
Product Specifications
Description
Approximately 7.8 kDa protein corresponding to 70 amino acid residues corresponding to CCL3/MIP-1 alpha, including the four highly conserved cysteine residues present in CC chemokines Source: E. coli
Uniprot ID: P10147
Amino Acid Sequence: ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of CCL3/MIP-1 alpha as determined by LAL method.
Predicted Molecular Mass
7.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
CCL3 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human CCL3/MIP-1 alpha Protein
![SDS-PAGE: Recombinant Human CCL3/MIP-1 alpha Protein [NBP2-35011]](https://resources.bio-techne.com/images/products/Human-CCL3-MIP-1-alpha-Protein-SDS-Page-NBP2-35011-img0001.jpg "SDS-PAGE: Recombinant Human CCL3/MIP-1 alpha Protein [NBP2-35011]")
SDS-PAGE: Recombinant Human CCL3/MIP-1 alpha Protein [NBP2-35011]
SDS-Page: Human CCL3/MIP-1 alpha Protein [NBP2-35011]Formulation, Preparation and Storage
NBP2-35011
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: CCL3/MIP-1 alpha
Alternate Names
G0S19-1, LD78a, MIP-1 alpha, MIP1 alpha, MIP1A, PAT 464.1, SCYA3, SIS-beta, MIP-1 alpha/CCL3
Gene Symbol
CCL3
UniProt
Additional CCL3/MIP-1 alpha Products
Product Documents for Recombinant Human CCL3/MIP-1 alpha Protein
Product Specific Notices for Recombinant Human CCL3/MIP-1 alpha Protein
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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