Recombinant Human CCL3/MIP-1 alpha Protein

Name: Recombinant Human CCL3/MIP-1 alpha Protein
Brand: Novus Biologicals
SKU: NBP2-35011

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35011

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P10147

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CCL3/MIP-1 alpha Proteins and Enzymes »

Product Specifications

Description

Approximately 7.8 kDa protein corresponding to 70 amino acid residues corresponding to CCL3/MIP-1 alpha, including the four highly conserved cysteine residues present in CC chemokines Source: E. coli

Uniprot ID: P10147

Amino Acid Sequence: ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL3/MIP-1 alpha as determined by LAL method.

Predicted Molecular Mass

7.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CCL3 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CCL3/MIP-1 alpha Protein

SDS-PAGE: Recombinant Human CCL3/MIP-1 alpha Protein [NBP2-35011]

SDS-Page: Human CCL3/MIP-1 alpha Protein [NBP2-35011]

Formulation, Preparation and Storage

NBP2-35011

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL3/MIP-1 alpha

CCL3, also known as macrophage inflammatory protein-1alpha (MIP-1alpha), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. Studies showed CCL3 can bind to CCR1, CCR4 and CCR5. MIP-1alpha can induce a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Recombinant human MIP-1alpha is a 7.8 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines and it shares approximately 73 %, 74 % and 92 % amino acid sequence homology with the murine, rat and rhesus macaque protein.

Alternate Names

G0S19-1, LD78a, MIP-1 alpha, MIP1 alpha, MIP1A, PAT 464.1, SCYA3, SIS-beta, MIP-1 alpha/CCL3

Gene Symbol

CCL3

UniProt