Recombinant Rabbit CCL3/MIP-1 alpha Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11066
Key Product Details
Product Specifications
Description
Purity
Predicted Molecular Mass
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Protein / Peptide Type
Scientific Data Images for Recombinant Rabbit CCL3/MIP-1 alpha Protein
ELISA: Recombinant Rabbit CCL3/MIP-1 alpha Protein [NBP3-11066]
ELISA: Recombinant Rabbit CCL3/MIP-1 alpha Protein [NBP3-11066] - Recombinant Rabbit MIP-1 Alpha detected using Chicken anti Rabbit MIP-1 Alpha as the capture reagent and Chicken anti Rabbit MIP-1 Alpha:Biotin as the detection reagent followed by Streptavidin:HRP.Formulation, Preparation and Storage
NBP3-11066
Preparation Method | Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography. |
Formulation | PBS |
Preservative | 10% Trehalose |
Concentration | Lyoph |
Reconstitution | Reconstitute with 20 uL sterile PBS containing 0.1% carrier protein. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Store at -20C. Avoid freeze-thaw cycles. |
Background: CCL3/MIP-1 alpha
MIP-1 alpha (macrophage inflammatory protein 1 alpha) is a member of the CC or beta chemokine subfamily that was originally purified from the conditioned media of an LPS-stimulated murine macrophage cell line. MIP-1 alpha acts as a chemoattractant to a variety of cells including monocytes, T cells, B cells and eosinophils.
The two human MIP-1 alpha genes arise by duplication/mutation. They code for MIP-1 alpha isoforms CCL3/LD78a and CCL3L1/LD78b, which share 94% amino acid sequence homology. Whereas the human CCL3/LD78a is a single-copy gene, the human CCL3L1/LD78b gene copy number varies within the population. Human CCL3L1/LD78b binds and signals through chemokine receptors CCR1, CCR5. When compared to CCL3/LD78a, CCL3L1/LD78b has higher binding affinity to CCR5, which also functions as a coreceptor for HIV-1 entry. The copy number of CCL3L1 is one of several genetic determinants of HIV-1 susceptibility.
Alternate Names
Gene Symbol
UniProt
Additional CCL3/MIP-1 alpha Products
Product Documents for Recombinant Rabbit CCL3/MIP-1 alpha Protein
Product Specific Notices for Recombinant Rabbit CCL3/MIP-1 alpha Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.