Recombinant Human delta-Sarcoglycan His Protein

Name: Recombinant Human delta-Sarcoglycan His Protein
Brand: Novus Biologicals
SKU: NBP2-23452

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23452

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all delta-Sarcoglycan Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 57-289 of Human delta-Sarcoglycan

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH RSHMKVMNFT IDGMGNLRIT EKGLKLEGDS EFLQPLYAKE IQSRPGNALY FKSARNVTVN ILNDQTKVLT QLITGPKAVE AYGKKFEVKT VSGKLLFSAD NNEVVVGAER LRVLGAEGTV FPKSIETPNV RADPFKELRL ESPTRSLVME APKGVEINAE AGNMEATCRT ELRLESKDGE IKLDAAKIRL PRLPHGSYTP TGTRQKVFEI CVCANGRLFL SQAGAGSTCQ INTSVCL

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

28.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human delta-Sarcoglycan His Protein

SDS-PAGE: Recombinant Human delta-Sarcoglycan His Protein [NBP2-23452]

SDS-Page: Recombinant Human delta-Sarcoglycan His Protein [NBP2-23452] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP2-23452

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: delta-Sarcoglycan

SGCD is one of the four known components of the sarcoglycan complex, which is a subcomplex of the dystrophin-glycoprotein complex (DGC). DGC forms a link between the F-actin cytoskeleton and the extracellular matrix. This protein is expressed most abundantly in skeletal and cardiac muscle. The mutations in this gene have been associated with autosomal recessive limb-girdle muscular dystrophy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding distinct isoforms have been observed. In melanocytic cells SGCD gene expression may be regulated by MITF. Recombinant human SGCD protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

35DAG, CMD1L, DAGD, deltaSarcoglycan, LGMD2F, SGCD

Gene Symbol

SGCD

Additional delta-Sarcoglycan Products

Product Documents for Recombinant Human delta-Sarcoglycan His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human delta-Sarcoglycan His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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