Recombinant Mouse FGF-21 Protein

Name: Recombinant Mouse FGF-21 Protein
Brand: Novus Biologicals
SKU: NBP2-35031

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35031

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Product Specifications
Scientific Data
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Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

Q9JJN1

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all FGF-21 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 182 amino acids corresponding to FGF-21 Source: E. coli

Uniprot ID: Q9JJN1

Amino Acid Sequence: AYPIPDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGAAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GALYGSPHFD PEACSFRELL LEDGYNVYQS EAHGLPLRLP QKDSPNQDAT SWGPVRFLPM PGLLHEPQDQ AGFLPPEPPD VGSSDPLSMV EPLQGRSPSY AS

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of FGF-21 as determined by LAL method.

Predicted Molecular Mass

19.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

FGF-21 protein is fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ug/ml, corresponding to a specific activity of > 2.0 x 10^3 IU/mg in the presence of 5 ug/ml of rMuKlotho-beta and 10 ug/ml of heparin.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse FGF-21 Protein

SDS-PAGE: Recombinant Mouse FGF-21 Protein [NBP2-35031]

SDS-Page: Mouse FGF-21 Protein [NBP2-35031]

Formulation, Preparation and Storage

NBP2-35031

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 3 x PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: FGF-21

Fibroblast growth factor-21 (FGF-21) belongs to the large FGF family and it is specifically induced by HMGCS2 activity. In mice, brown adipose tissue becomes a source of systemic FGF21 after cold exposure. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. FGF-21, in the presence of betaKlotho as a protein cofactor, signals through the FGFR 1c and 4 receptors. Recombinant murine FGF-21 contains 182 amino acids residues and show limited binding to heparin. In addition, Murine FGF-21 respectively shows 81 % and 92 % a.a. identity to human and rat FGF-21, and it show activity on human and rat cells.

Long Name

Fibroblast Growth Factor 21

Alternate Names

FGF21

Gene Symbol

fgf21

UniProt