Recombinant Human FGF-21 Protein

Name: Recombinant Human FGF-21 Protein
Brand: Novus Biologicals
SKU: NBP2-35023

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35023

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Product Specifications
Scientific Data
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Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

Q9NSA1

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all FGF-21 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 181 amino acids corresponding to FGF-21 Source: E. coli

Uniprot ID: Q9NSA1

Amino Acid Sequence: HPIPDSSPLL QFGGQVRQRY LYTDDAQQTE AHLEIREDGT VGGAADQSPE SLLQLKALKP GVIQILGVKT SRFLCQRPDG ALYGSLHFDP EACSFRELLL EDGYNVYQSE AHGLPLHLPG NKSPHRDPAP RGPARFLPLP GLPPALPEPP GILAPQPPDV GSSDPLSMVG PSQGRSPSYA S

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of FGF-21 as determined by LAL method.

Predicted Molecular Mass

19.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

FGF-21 protein is fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ug/ml, corresponding to a specific activity of > 2.0 x 10^3 IU/mg in the presence of 5 ug/ml of rMuKlotho-beta and 10 ug/ml of heparin.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human FGF-21 Protein

SDS-PAGE: Recombinant Human FGF-21 Protein [NBP2-35023]

SDS-Page: Human FGF-21 Protein [NBP2-35023]

Formulation, Preparation and Storage

NBP2-35023

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: FGF-21

Fibroblast growth factor-21 (FGF-21) belongs to the large FGF family which is encoded by the FGF-21 gene and it is specifically induced by HMGCS2 activity. All FGF family members are heparin binding growth factors with a core 120 amino acid (a.a.) FGF domain that allows for a common tertiary structure and they are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. FGF-21 contains a 28 a.a. signal sequence and a 181 a.a. mature region but show limited binding to heparin. In addition, Mature human FGF-21 respectively shows 81 % a.a. identity to murine and rat FGF-21, and is known to be active on murine cells.

Long Name

Fibroblast Growth Factor 21

Alternate Names

FGF21

Gene Symbol

fgf21

UniProt