Recombinant Rat FGF-21 Protein

Name: Recombinant Rat FGF-21 Protein
Brand: Novus Biologicals
SKU: NBP2-35194

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35194

Submit a review

Product Datasheet / COA

Catalog #

Availability

Size / Price

Qty

100% Guarantee

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

Q8VI80

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all FGF-21 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 180 amino acids corresponding to FGF-21 Source: E. coli

Uniprot ID: Q8VI80

Amino Acid Sequence: AYPISDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGTAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GTLYGSPHFD PEACSFRELL LKDGYNVYQS EAHGLPLRLP QKDSQDPATR GPVRFLPMPG LPHEPQEQPG VLPPEPPDVG SSDPLSMVEP LQGRSPSYAS

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of FGF-21 as determined by LAL method.

Predicted Molecular Mass

19.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

FGF-21 protein is fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ug/ml, corresponding to a specific activity of > 2.0 x 10^3 IU/mg in the presence of 5 ug/ml of rMuKlotho-beta and 10 ug/ml of heparin.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat FGF-21 Protein

SDS-PAGE: Recombinant Rat FGF-21 Protein [NBP2-35194]

SDS-Page: Rat FGF-21 Protein [NBP2-35194]

Formulation, Preparation and Storage

NBP2-35194

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, 500 mM NaCl, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: FGF-21

Fibroblast growth factor 21 (FGF-21) encoded by the FGF-21 gene belongs to the large FGF family and it is specifically induced by HMGCS2 activity. In mice, brown adipose tissue becomes a source of systemic FGF-21 after cold exposure. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. Recombinant rat FGF-21 contains 180 amino acids residues and show limited binding to heparin. In addition, rat FGF-21 respectively shows 81 % and 92 % a.a. identity to human and murine FGF-21, and it show activity on murine and human cells.

Long Name

Fibroblast Growth Factor 21

Alternate Names

FGF21

Gene Symbol

fgf21

UniProt

Q8VI80 (Rat)

Additional FGF-21 Products

Product Documents for Recombinant Rat FGF-21 Protein

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for Recombinant Rat FGF-21 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations
Reviews