Recombinant Human FGF acidic/FGF1 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35052
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 141 amino acids corresponding to FGF acidic/FGF1 Source: E. coli
Uniprot ID: P05230
Amino Acid Sequence: MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of FGF acidic/FGF1 as determined by LAL method.
Predicted Molecular Mass
16.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
FGF acidic/FGF1 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.5 ng/ml, corresponding to a specific activity of >2.0 x 10^6 IU/mg in the presence of 10 ug/ml of heparin.
Protein / Peptide Type
Recombinant Protein
Reviewed Applications
Read 1 review rated 5 using NBP2-35052 in the following applications:
Scientific Data Images for Recombinant Human FGF acidic/FGF1 Protein
![SDS-PAGE: Recombinant Human FGF acidic/FGF1 Protein [NBP2-35052]](https://resources.bio-techne.com/images/products/Human-FGF-acidic-FGF1-Protein-SDS-Page-NBP2-35052-img0001.jpg "SDS-PAGE: Recombinant Human FGF acidic/FGF1 Protein [NBP2-35052]")
SDS-PAGE: Recombinant Human FGF acidic/FGF1 Protein [NBP2-35052]
SDS-Page: Human FGF acidic/FGF1 Protein [NBP2-35052]Formulation, Preparation and Storage
NBP2-35052
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: FGF acidic/FGF1
Long Name
Fibroblast Growth Factor acidic
Alternate Names
alpha-ECGF, beta-ECGF, FGF acidic, FGF-1, HBGF-1
Gene Symbol
FGF1
UniProt
Additional FGF acidic/FGF1 Products
Product Documents for Recombinant Human FGF acidic/FGF1 Protein
Product Specific Notices for Recombinant Human FGF acidic/FGF1 Protein
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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