Recombinant Human FGF acidic/FGF1 Protein

Name: Recombinant Human FGF acidic/FGF1 Protein
Brand: Novus Biologicals
SKU: NBP2-61310

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61310

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, Western Blot

View all FGF acidic/FGF1 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 154 amino acids corresponding to FGF acidic/FGF1 Source: E. coli

Uniprot ID: P05230

Amino Acid Sequence: AEGEITTFTA LTEKFNLPPG NYKKPKLLYC SNGGHFLRIL PDGTVDGTRD RSDQHIQLQL SAESVGEVYI KSTETGQYLA MDTDGLLYGS QTPNEECLFL ERLEENHYNT YISKKHAEKN WFVGLKKNGS CKRGPRTHYG QKAILFLPLP VSSD

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of FGF acidic/FGF1 as determined by LAL method.

Predicted Molecular Mass

17.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

FGF acidic/FGF1 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg in the presence of 10 ug/ml of heparin.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human FGF acidic/FGF1 Protein

SDS-PAGE: Recombinant Human FGF acidic/FGF1 Protein [NBP2-61310]

SDS-Page: Recombinant Human FGF acidic/FGF1 Protein [NBP2-61310]

Formulation, Preparation and Storage

NBP2-61310

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 2 mM EDTA, 0.5 mM DTT.
Preservative	5% Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: FGF acidic/FGF1

FGF acidic, also known as FGF-1, ECGF, and HBGF-1, is a secreted mitogen that stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. FGF acidic is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate. The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors. Internalized FGF acidic can translocate to the cytosol and to the nucleus. Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling.

Long Name

Fibroblast Growth Factor acidic

Alternate Names

alpha-ECGF, beta-ECGF, FGF acidic, FGF-1, HBGF-1

Gene Symbol

FGF1

Additional FGF acidic/FGF1 Products

Product Documents for Recombinant Human FGF acidic/FGF1 Protein

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COA

Product Specific Notices for Recombinant Human FGF acidic/FGF1 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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