Recombinant Rat FGF basic/FGF2/bFGF Protein

Name: Recombinant Rat FGF basic/FGF2/bFGF Protein
Brand: Novus Biologicals
SKU: NBP2-35239

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35239

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Product Specifications
Scientific Data
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Key Product Details

Source

E. coli

Accession #

P13109

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all FGF basic/FGF2/bFGF Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 146 amino acids corresponding to FGF basic/FGF2/bFGF Source: E. coli

Uniprot ID: P13109

Amino Acid Sequence: MPALPEDGGG AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of FGF basic/FGF2/bFGF as determined by LAL method.

Predicted Molecular Mass

16.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

FGF basic/FGF2/bFGF protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.2 ng/ml, corresponding to a specific activity of >5.0 x 10^6 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat FGF basic/FGF2/bFGF Protein

SDS-PAGE: Recombinant Rat FGF basic/FGF2/bFGF Protein [NBP2-35239]

SDS-Page: Rat FGF basic/FGF2 Protein [NBP2-35239]

Formulation, Preparation and Storage

NBP2-35239

Formulation	Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: FGF basic/FGF2/bFGF

Rat bFGF, encoded by the FGF2 gene, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic pH, two different forms have isolated that named "acidic fibroblast growth factor" (FGF-1) and "basic fibroblast growth factor" (FGF-2). Rat bFGF shares 96 % amino acid sequence identity with human bFGF, and 98 % amino acid sequence identity with mouse. Affinity between bFGF and its receptors can be increased by heparin or heparan sulfate proteoglycan. bFGF plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. bFGF are also involved in a variety of biological processes, including embryonic development, morphogenesis, tissue repair, tumor growth and invasion. Additionally, bFGF is frequently used for a critical component of cell culture medium, e.g., human embryonic stem cell culture medium, serum-free culture systems.

Long Name

Fibroblast Growth Factor basic

Alternate Names

bFGF, FGF-2, FGF2, HBGF-2, Prostatropin

Gene Symbol

FGF2

UniProt