Recombinant Human Glucagon His Protein

Name: Recombinant Human Glucagon His Protein
Brand: Novus Biologicals
SKU: NBP1-72384

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72384

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Technical Data
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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Glucagon Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 90-180 of Human Glucagon

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MKRHDEFERH AEGTFTSDVS SYLEGQAAKE FIAWLVKGRG RRDFPEEVAI VEELGRRHAD GSFSDEMNTI LDNLAARDFI NWLIQTKITD RK

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

12.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Glucagon His Protein

Recombinant Human Glucagon Protein [NBP1-72384]

Formulation, Preparation and Storage

NBP1-72384

Formulation	20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 50% glycerol, 0.2 M NaCl
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glucagon

Glucagon, also known as GCG, a hormone secreted by the pancreas, raises blood glucose levels. Its effect is opposite that of insulin, which lowers blood glucose levels. The pancreas releases glucagon when blood sugar (glucose) levels fall too low. Glucagon causes the liver to convert stored glycogen into glucose, which is released into the bloodstream. Glucagon also stimulates the release of insulin, so glucose can be taken up and used by insulin-dependent tissues. Thus, glucagon and insulin are part of a feedback system that keeps blood glucose levels at a stable level. Recombinant human GCG protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

GCG, GRPP

Gene Symbol

GCG

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COA

Product Specific Notices for Recombinant Human Glucagon His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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