I am looking for the recombinant human HMGB-1 which we would like to use for cultured human intervertebral disc cells in vitro. 1)Is your HMGB-1 protein NBC1-18488 already sterilized? 2)Are there some articles in which NBC1-18488 is used for human cultured cells in vitro?

NBC1-18488 is not sterilized, and may not be suitable for use in live cells. We have only approved this protein to work for ELISA and western blot, and have not assessed its biological activity. As such, we do not have any references that cite this product in cell culture.

Are both of these proteins any carrier protein free? HMG1L10 Protein (Catalog # P3789) and HMGB1 Protein (catalog # NBC1-18488)

These proteins are carrier free. Catalog # P3789 is supplied in 50 mM HEPES, 100mM NaCl, pH 7.5 (30% glycerol, 30 mM Glutathione, 0.5% TritonX-100, 1 mM dithiothreitol). Catalog #NBC1-18488 is supplied in liquid in 20mM Tris pH 8.0, 1mM EDTA, 0.5mM DTT, 10% glycerol.

Recombinant Human HMGB1/HMG-1 His Protein

Name: Recombinant Human HMGB1/HMG-1 His Protein
Brand: Novus Biologicals
SKU: NBC1-18488

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-18488

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

Baculovirus

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all HMGB1/HMG-1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-215 of Human HMGB1/HMG-1

Source: Baculovirus

Amino Acid Sequence: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDELEHHH HHH

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human HMGB1/HMG-1 His Protein

SDS-PAGE: Recombinant Human HMGB1/HMG-1 His Protein [NBC1-18488]

SDS-Page: Recombinant Human HMGB1/HMG-1 Protein [NBC1-18488] - HMGB1, 25kDa (223aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE.

Formulation, Preparation and Storage

NBC1-18488

Formulation	20 mM Tris-HCl buffer (pH 8.0), 1 mM EDTA, 0.5 MM DTT, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HMGB1/HMG-1

High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor. Recombinant human HMGB1 was expressed in High Five insect cells and purified by conventional chromatography techniques.

Long Name

High Mobility Group Protein 1

Alternate Names

HMG-1, HMG1

Gene Symbol

HMGB1

Additional HMGB1/HMG-1 Products

Product Documents for Recombinant Human HMGB1/HMG-1 His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human HMGB1/HMG-1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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