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Recombinant Mouse IL-28A/IFN-lambda 2 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35074

Novus Biologicals, part of Bio-Techne
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NBP2-35074-100ug
NBP2-35074-1mg
NBP2-35074-500ug
NBP2-35074-5ug

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 174 amino acids corresponding to IL-28A/IFN-lambda 2 Source: E. coli

Uniprot ID: Q4VK74

Amino Acid Sequence: DPVPRATRLP VEAKDCHIAQ FKSLSPKELQ AFKKAKDAIE KRLLEKDMRC SSHLISRAWD LKQLQVQERP KALQAEVALT LKVWENMTDS ALATILGQPL HTLSHIHSQL QTCTQLQATA EPKPPSRRLS RWLHRLQEAQ SKETPGCLED SVTSNLFRLL TRDLKCVASG DQCV

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of IL-28A/IFN-lambda 2 as determined by LAL method.

Predicted Molecular Mass

19.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL28A Protein is fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis is less than 3 ng/ml, corresponding to a specific activity of > 3.3 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse IL-28A/IFN-lambda 2 Protein

SDS-PAGE: Recombinant Mouse IL-28A/IFN-lambda 2 Protein [NBP2-35074]

SDS-PAGE: Recombinant Mouse IL-28A/IFN-lambda 2 Protein [NBP2-35074]

SDS-Page: Mouse IL-28A/IFN-lambda 2 Protein [NBP2-35074]

Formulation, Preparation and Storage

NBP2-35074
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative 5% Trehalose
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-28A/IFN-lambda 2

Interferon lambda-2, as known as IL-28A, belongs to the lambda interferon family. IL-28A (IFN-lambda2), IL-28B (IFN-lambda3) and IL-29 (IFN-lambda1) are type III interferons that are class II cytokine receptor ligands. These IFNs signal through a receptor complex consisting of IL10R2 (also called CRF2-4) and IFNLR1 (also called CRF2-12). Interferon lambda-2 displays potent antiviral activity and antitumor activity, so it plays a significant role in the antiviral immune defense in the intestinal epithelium. It also upregulate MHC class I antigen expression. Recombinant murine interferon lambda-2 contains 174 amino acids and it shares 81 % and 66 % a.a. sequence identity with rat and human IFN-lambda2, respectively, and functions across species.

Long Name

Interleukin 28A

Alternate Names

IFN-lambda 2, IL28A, IFN-lambda 2/IL-28A

Gene Symbol

IFNL2

Additional IL-28A/IFN-lambda 2 Products

Product Documents for Recombinant Mouse IL-28A/IFN-lambda 2 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Mouse IL-28A/IFN-lambda 2 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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